John Tuttle graduated from the Curtis Institute where he studied organ with Alexander McCurdy. Following graduation he served in the United States Army as Organist and Choirmaster of the Post Chapel at theUnited States Military Academy , West Point , NY , and then returned in 1971 to serve as Dr. McCurdy's successor at the First Presbyterian Church in Philadelphia .
In 1975 he left the United States for Toronto, Canada , to become Organist and Choirmaster of St. Paul’s Anglican Church, where he remained until accepting the post of Organist and Choirmaster for St. Thomas ’s Anglican Church in 1989.In 1979 he was appointed Organist to the University of Toronto, where he also teaches organ for the Faculty of Music. In 1981 he founded the Exultate Chamber Singers, a twenty-voice ensemble that has gained a national reputation for superb choral singing. He retired from conducting the Exultate Chamber Singers in May 2011. From 1981 to 2006 he conducted the Hart House Chorus. From 1985 to 2000 he was Music Director of the Canadian Children's Opera Chorus, which commissioned numerous operas for children, and toured throughout Canada and the USA.
In 2005 he accepted the post of Organist and Choirmaster of Trinity College Chapel at the University, where he trains the Trinity Chapel Choir for weekly services, works with the Bevan Organ Scholar, and coaches the Divinity students in liturgical music.
He holds the Fellowship Diploma of the American Guild of Organists, and holds honorary Fellowship diplomas from the Royal College of Organists (UK) and the Royal Canadian College of Organists, of which he was Naional President from 1986 to 1988.
In 2004 he was awarded a Doctor of Sacred Letters (honoris causa) from Trinity College.